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OBJECTIVE: To identify the poor working posture on musculoskeletal disorders of workers in greenhouse vegetable plantation(hereinafter referred to as “vegetable workers”) in different planting stages, and to evaluate the risk grade of working posture. METHODS: A total of 28 vegetable workers in a greenhouse vegetable planting base in Shandong Province were taken as research subjects using convenient sampling method. Video data were collected from field observation. Working posture analysis system was used to code the working posture of vegetable workers. The composition of working posture was analyzed, and the risk grade of working posture was evaluated. RESULTS: In the planting and seedling period, the main poor working postures of workers were lumbar back leaning forward, legs squatting, lumbar back bending, and legs bending, and the composition ratios were 60.4%, 42.9%, 38.6% and 38.4% respectively. In the management period, the main poor working postures were neck forward, waist back bending, hands higher than elbows, and the composition ratios were 52.0%, 34.2% and 30.0% respectively. In the harvest period, the main poor working postures were neck leaning forward, one hand above elbow, lumbar back bending, walking and lumbar back leaning, and the composition ratios were 50.4%, 42.6%, 39.6%, 39.1% and 35.4% respectively. In the period of planting and seedling, management and harvest, there were 81.6%, 23.4% and 52.9% of the work position in the risk grade Ⅲ; there was 1.4% of the work position in the risk grade Ⅳ in the harvest period. CONCLUSION: Working postures with obvious hazards existed at different planting periods. Effective intervention measures should be taken to prevent the problems of poor working posture in greenhouse workers.
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Objective To express UL148 RNA of human cytomegalovirus (HCMV) clinical strains in vitro and to study its functions. Methods Urine of a newborn with HCMV infection was inocula-ted into human embryo lung cells. HCMV clinical strain was isolated and identified by multiplex PCR. UL148 gene was amplified and cloned into pGEM-T-Easy plasmid after double enzyme digestion. A recombi-nant plasmid was constructed and located at the downstream of the T7 promoter. The recombinant plasmid was identified by electrophoresis of the recombinant plasmid,PCR product and double enzyme product. Se-quencing analysis was used for final confirmation. UL148 was transcribed into RNA by 32P labeling. Post-translational modification sites were analyzed by bioinformatics method based on UL148 sequence characteris-tics. Results The clinical strain of HCMV was obtained in vitro. Electrophoresis and sequencing analysis confirmed the successful construction of the recombinant plasmid. UL148 RNA was transcribed in vitro by T7RNA polymerase. Post-translational modification sites showed that UL148 gene contained one cell adhe-sion sequence, one legume lectins beta-chain signature, two N-myristoylation sites, one casein kinase Ⅱphosphorylation site,seven protein kinase C phosphorylation sitse, one cAMP/cGMP-dependent protein ki-nase phosphorylation site, two N-glycosylation sites and one transmembrane region. Conclusion UL148 gene might encode a viral adhesion molecule involving in the signal transduction in host cells.
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Objective@#To investigate the UL148 gene function of human cytomegalovirus (HCMV) low passage clinic isolate and new strategies for anti-HCMV treatment, the DNA-based external guide sequences (EGSs) were designed to inhibit UL148 RNA expression.@*Methods@#UL148 RNA secondary structure was analyzed by RNA structure technique, an appropriate region was chosen for DNA-based EGS57 synthesis, targeted the UL148 RNA. The M1RNA and UL148 RNA were generated by PCR for transcription in vitro. The UL148 RNA and M1RNA were transcribed in vitro under the function of T7 RNA polymerase. The UL148 was labelled by 32P. The cleavage reactions were carried out by mixing up EGS, M1RNA with UL148 RNA for 1 h. The products were separated by urea denaturing polyacrylamide gel electrophoresis and detected with Typhoon Phosphor Imager.@*Results@#UL148 RNA ranged from 58 to 72 sites was the binding position, and 57 was a cleavage site. EGS57 was designed and synthesized. EGS57 was combined with UL148 RNA to form the natural substrate of M1RNA. UL148 RNA and M1RNA were synthesized through T7 RNA polymerase catalyzing, and the products were conformed. After cleaving reactions, DNA-based EGS57 was shown to be able to cleave UL148 RNA efficiently in vitro by a complex with M1RNA.@*Conclusions@#UL148 RNA was cleaved efficiently by EGS57, and the cleaving site was conformed as expectation. It will provide the gene silent tool effectively for further study the function of UL148 gene.
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Work-related musculoskeletal disorders (MSDs) are most commonly seen in all the occupational non-fatal injuries and illnesses for workers,especially those who are involved in labor-intensive industries.Participatory ergonomics is frequently used to prevent musculoskeletal disorders.This paper gives an overview of a historical perspective on the use of participatory ergonomics approach in reducing the health effects of labor-intensive industries.Progress,barriers and facilitators on the organization,implementation and evaluation of participatory ergonomics programs are studied.Participatory ergonomics seems a successful method to develop,prioritize measures to prevent MSDs.Participatory ergonomics can help industries reduce musculoskeletal injuries and disorders,improve workplace condition and promote health conditions of the workers.
ABSTRACT
Work-related musculoskeletal disorders (MSDs) are most commonly seen in all the occupational non-fatal injuries and illnesses for workers,especially those who are involved in labor-intensive industries.Participatory ergonomics is frequently used to prevent musculoskeletal disorders.This paper gives an overview of a historical perspective on the use of participatory ergonomics approach in reducing the health effects of labor-intensive industries.Progress,barriers and facilitators on the organization,implementation and evaluation of participatory ergonomics programs are studied.Participatory ergonomics seems a successful method to develop,prioritize measures to prevent MSDs.Participatory ergonomics can help industries reduce musculoskeletal injuries and disorders,improve workplace condition and promote health conditions of the workers.
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Objective To analyze the nucleotide sequences and genetic polymorphisms of UL138 gene of low passage human cytomegalovirus ( HCMV) strains isolated from infants in Guangzhou province. Methods The low passage strains of HCMV were isolated from urine samples of 10 infants with HCMV in-fection in Guangzhou province and identified by multiplex PCR.The UL138 genes were amplified, cloned and identified with sequencing.The sequences were analyzed together with the homologous sequences of 10 clinical isolates published in GenBank.The sequences of UL138 genes were analyzed by using bioinformatics softwares for investigation of the post-translational modification sites, isoelectric points and second structures of UL138 proteins.Results Three low passage strains of HCMV ( D2, D3 and D52) were isolated from in-fants with congenital HCMV infection.The complete sequences of UL138 genes of the three strains were sub-mitted to GenBank after sequencing identification with the GenBank accession numbers of DQ180375, DQ180387 and DQ180359, respectively.The UL138 gene sequences of the three clinical isolates were high-ly conservative.Among the 841 base pairs of the UL138 gene sequences, mutations were identified in 16 sites with base substitution, no any insertion and deletion mutation was found.The 16 mutations resulted in 7 amino acid changes.No additional or deleted sites were found with regard to the post translational modifi-cation sites of UL138 protein in all clinical isolates except the Toledo strain.The isoelectric point of UL138 protein was 6.51 for all clinical isolates.Conclusion The UL138 genes and the deduced amino acid se-quences of HCMV strains isolated from infants in Guangzhou were highly conservative, regardless of the poly-morphism of UL138 gene.This study paved the way for further investigation on HCMV infection and its path-ogenic mechanism.